Hematopoiesis is severely altered in mice with an induced osteoblast deficiency. Continuous cell supply from Krt7-expressing hematopoietic stem cells during native hematopoiesis revealed by targeted in vivo gene transfer method. Fgd5 identifies hematopoietic stem cells in the murine bone marrow. Self-renewal of a purified Tie2 + hematopoietic stem cell population relies on mitochondrial clearance. This study systematically images the Scf expression pattern in the bone marrow then conditionally deletes Scf from candidate niche cells to show that HSCs depend on SCF synthesized by endothelial cells and LEPR + stromal cells. Endothelial and perivascular cells maintain haematopoietic stem cells. Sl/Sl d hematopoietic progenitors are deficient in situ. This study identifies a new HSC marker, Hoxb5, the expression of which is highly restricted among haematopoietic cells to HSCs and enables the imaging of HSC localization in the bone marrow.īarker, J. Hoxb5 marks long-term haematopoietic stem cells and reveals a homogenous perivascular niche. This paper uses optical clearing and a new HSC marker, α -catulin, to localize HSCs throughout large segments of the bone marrow by deep imaging and digital reconstruction.Ĭhen, J. Deep imaging of bone marrow shows non-dividing stem cells are mainly perisinusoidal. This paper uses confocal imaging and spatial modelling to show that although most HSCs are closest to sinusoidal blood vessels, some HSCs are more closely associated with arterioles.Īcar, M. Arteriolar niches maintain haematopoietic stem cell quiescence. Maintenance of the hematopoietic stem cell pool by CXCL12–CXCR4 chemokine signaling in bone marrow stromal cell niches. Quantitative imaging of haematopoietic stem and progenitor cell localization and hypoxic status in the bone marrow microenvironment. SLAM family receptors distinguish hematopoietic stem and progenitor cells and reveal endothelial niches for stem cells. The bone marrow niche for haematopoietic stem cells. The relationship between the spleen colony-forming cell and the haemopoietic stem cell. Allogeneic haematopoietic stem cell transplantation: individualized stem cell and immune therapy of cancer. Exit from dormancy provokes DNA-damage-induced attrition in haematopoietic stem cells. Hematopoietic stem cells are the major source of multilineage hematopoiesis in adult animals. Diverse and heritable lineage imprinting of early haematopoietic progenitors. Fundamental properties of unperturbed haematopoiesis from stem cells in vivo. Clonal dynamics of native haematopoiesis. Stem cells and niches: mechanisms that promote stem cell maintenance throughout life. The vascular and stromal compositions of the bone marrow change during ageing. This niche is necessary for the recovery of haematopoiesis from haematopoietic stresses such as blood loss. Several other cell types - including megakaryocytes, monocytes and macrophages, neurons (specifically, nerve fibres) and Schwann cells - directly or indirectly regulate HSC or niche function through other mechanisms.Įxtramedullary haematopoiesis in the spleen depends on a perivascular niche that is associated with sinusoids in the red pulp, in which endothelial cells and transcription factor 21-expressing stromal cells are the main sources of SCF and CXCL12. Other perivascular cells, such as Ng2-CreER + periarteriolar cells (which express neural–glial antigen 2), may or may not also synthesize the CXCL12 required for HSC maintenance. The periarteriolar and perisinusoidal microenvironments differ in terms of the capacity of HSCs to intravasate into the circulation and in terms of their exposure to blood plasma components.Įndothelial cells and leptin receptor-expressing, CXC-chemokine ligand 12 (CXCL12)-abundant reticular perivascular stromal cells are the main sources of the stem cell factor (SCF) and CXCL12 required for HSC maintenance in normal young-adult bone marrow. Dividing and non-dividing haematopoietic stem cells (HSCs) reside in perivascular niches that are mainly associated with sinusoidal blood vessels in adult bone marrow and spleen.Ī subset of HSCs is most closely associated with arterioles.
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